Optimal Primer Length:
(AGTC content + AEGIS spans) |
nts |
| Shortest Acceptable Primer: | nts |
| Longest Acceptable Primer: | nts |
|
|
| AEGIS Span Tm: | | | °C |
|
| Optimal AEGIS Bases Per Span: | nts |
| Minimum AEGIS Bases Per Span: | nts |
| Max Bases Between AEGIS and 3' End: | nts |
| Avoid 3' AEGIS Ends: | |
| Overhang Ends: |
|
| Circularize Sequence: | |
| AEGIS Bases To Use: |
|
| # of Synthesis Units: |
|
| Primer Concentration (for Tm): |
nM
|
| Salt Concentration (for Tm): |
mM
|
| Mg Concentration (for Tm): |
mM
|
Sequence to Design:
|
Protein-Coding Regions:
Help me choose.
Example format:
301-900
1500-1201
|
Sequence-Dependent Regions (promoters, replication origins, etc.):
Help me choose.
Example format:
50-75
4700-4900
|
Non-Changeable Regions (overhangs, restriction enzymes sites, etc.):
Help me choose.
Example format:
50-56
5002-5005
|